Effect of Solvent System on Extractability of Lipidic Components of Scenedesmus obliquus (M2-1) and Gloeothece sp. on Antioxidant Scavenging Capacity Thereof
نویسندگان
چکیده
Microalgae are well known for their biotechnological potential, namely with regard to bioactive lipidic components-especially carotenoids and polyunsaturated fatty acids (PUFA), well-known for therapeutic applications based on their antioxidant capacity. The aim of this work was to evaluate the influence of four distinct food-grade solvents upon extractability of specific lipidic components, and on the antioxidant capacity exhibited against both synthetic (2,2-diphenyl-1-picrylhydrazyl (DPPH(•)) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS(+•))) and biological reactive species (O₂(•)⁻ and (•)NO⁻). A eukaryotic microalga (Scenedesmus obliquus (M2-1)) and a prokaryotic one (Gloeothece sp.) were used as case studies. Concerning total antioxidant capacity, the hexane:isopropanol (3:2) and acetone extracts of Sc. obliquus (M2-1) were the most effective against DPPH(•) and ABTS(+•), respectively. Gloeothece sp. ethanol extracts were the most interesting scavengers of O₂(•)⁻, probably due the high content of linolenic acid. On the other hand, acetone and hexane:isopropanol (3:2) extracts were the most interesting ones in (•)NO⁻ assay. Acetone extract exhibited the best results for the ABTS assay, likely associated to its content of carotenoids, in both microalgae. Otherwise, ethanol stood out in PUFA extraction. Therefore, profiles of lipidic components extracted are critical for evaluating the antioxidant performance-which appears to hinge, in particular, on the balance between carotenoids and PUFAs.
منابع مشابه
Effect of Drought Stress on Chemical Constituents, Photosynthesis and Antioxidant Properties of Rosmarinus officinalis Essential Oil
This research was carried out to assess the effect of drought stress on head branch dry weight, photosynthesis, essential oil yield and radical scavenging of rosemary (Rosmarinus officinalis L.) in Islamic Azad University, Shoushtar branch, Iran during 2010-2011.The experiments were carried out using complete randomized block design with four replications and drought stress levels included cont...
متن کاملAccumulation of Cu by Microalgae Scenedesmus obliquus and Synechocystis sp. PCC 6803
This study focused on the use of microbial potential for metal ion removal. Scenedesmus obliquus and Synechocystis sp. PCC 6803 were compared for accumulation of Cu in batch mode. During time course accumulation, S. obliquus accumulated Cu up to 124 μg mg -1 dry wt after 1h of incubation, whereas for Synechocystis sp. PCC 6803 it reached up to 64.9 μg mg -1 dry wt after 2h. Low biomass concentr...
متن کاملنقش افزایش قطبیت حلال در محتوای تام فنل و فلاونوئیدی و میزان فعالیت آنتی اکسیدانی گیاه مورد Myrtus communis L.
Background and purpose: Medicinal plants are the main source of very potent antioxidants. Myrtus communis L. (Myrtle, Myrtaceae) is a medicinal plant used worldwide in traditional medicine. It is a well-known plant with distinctive antioxidant activity. This study aimed at investigating the effect of solvents extraction on total phenolics and antioxidant activity of extracts from Myrtle (Myrt...
متن کاملEvaluation of the Antioxidant Activity of Cell Extracts from Microalgae
A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both w...
متن کاملThe effect of immobilization of Scenedesmus sp. ISC 109 on potential of reduction of Chromium
Recently, biological methods are used for remediation of various pollutants especially heavy metals. Algae are organisms which can accumulate and transform these pollutants with different mechanisms. In this regard immobilization of these microorganisms is a technique that is used for different purpose. So after primary screening of microalgae (Spirulina, Tetraselmis, Chlorella, Scenedesmus, Mi...
متن کامل